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4A), and we note that a small proportion of genes, detected as asymmetrically distributed in the pooled data, were not detected as such in any embryo on its own

Online PR News – 06-November-2017 – WA – 4A), and we note that a small proportion of genes, detected as asymmetrically distributed in the pooled data, were not detected as such in any embryo on its own (Table 1). For a given gene the measured fold change varied between embryos: summarizing over all genes found asymmetric at the pooled level, we found that ~93% of the fold changes found in individual embryos are within 2-fold of the fold change found in the pooled embryo tests. The variation in fold-change, and the differences between the poles, can be click here seen clearly in the distribution of fold-changes over all asymmetric genes for each individual embryo (Fig. 4B). The range of fold-change is always greater on the vegetal pole side, with roughly the same number of genes compressed into a narrower range on the animal side. We report the range of fold changes from individual embryos in the tabulated lists of differentially expressed genes (Table 2 and Supplementary file 3) as well as the number of individual embryos in which they were found significantly differentially expressed. That we do not find significant differential expression in all embryos at the single embryo level (for genes found asymmetric at the pooled level), is a consequence of the lack of statistical power at this level, and not the lack of asymmetry. In fact we find that, in 95% of those cases where the significance fell below our (FDR

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Dimitri Grantham
Allie
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